Page 14 - 10-BIO-23-newflip

23.Biotechnology eLearn.Punjab

For generation of different sized DNA fragments, two methods arc generally used. One
is Sanger’s,method in which dideoxyribonucleoside triphosphates arc used to terminate
DNA synthesis at different sites. The other method is known as Maxam-Giibcrt method
in which DNA threads are chemically cut into pieces of different sizes

Fig 23.8 The enzymatic or dideoxy method of sequencing DNA

14